FastQCFastQC Report
Wed 23 Nov 2022
HCJ57BGXN_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJ57BGXN_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389501610
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG41399601.0628864923048713RNA PCR Primer, Index 41 (96% over 30bp)
GCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGGG28047070.7200758425619858RNA PCR Primer, Index 41 (96% over 30bp)
GCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGGG6367600.16348071064455932RNA PCR Primer, Index 41 (96% over 30bp)
GCAGGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGTGGGGG5317680.1365252379829701RNA PCR Primer, Index 41 (96% over 30bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)