FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47344099
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1455060.3073371403688557No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG589700.12455617752911508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTG78500.054.9251066
ACGCACT103300.054.5449945
CGCACTA74200.052.1180576
GCGCACT42850.045.3285185
CCGCACT56450.043.5213665
GCACTGT79750.041.33817
TCGCACT64950.040.5737535
CGCACTC66700.040.1388976
TAAACTG190950.039.7163286
CGCACTT47650.038.0449186
TACGCAC42900.035.2418024
GTAAACT161200.034.1284755
AACGCAC81050.033.1187554
CACTGTA89050.032.501048
TAAACTA414700.030.7858126
AACTGTA143950.030.1464168
CTGTAGA225100.028.66902510
ATAAACT655000.026.8594425
TGTAGAA233400.026.47996511
TGCGCAC21400.026.0024954
Produced by FastQC (version 0.11.5)