FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_pIXHALO_Vv_B_h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_pIXHALO_Vv_B_h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25748930
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGCG36450.021.31882541
CATCGGG119350.021.05764641
CGCATCG163500.020.93745239
GCGCATC198300.020.74047938
GCATCGG128200.020.0677940
GCGGGGT41300.019.83005152
ATCGGGG97200.019.41002542
ATCGCGT15500.019.19511842
TGGCGGG37600.019.08193250
TGCGCAT233550.018.70387537
ATCGCGG26600.017.50143242
CGGCGTG13600.017.49952953
CGGGGTG47600.017.42653
ATGCGCA265100.017.28316936
GCGGCGT12750.016.4702152
GCATCGC50750.016.34603940
TGCGGGG29650.016.17166551
CGGACTG17500.015.9992586
AATGCGC297500.015.98954435
TTGCGGG35050.015.77703750
Produced by FastQC (version 0.11.5)