FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_pIXHALO_F1b_B_h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_pIXHALO_F1b_B_h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1610935
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG4550.032.310542
TCGGGGG5200.028.27168743
CGGGGGG4650.024.08736844
CGGGGGT1601.8644641E-924.06384544
AATCGGG6750.022.29823341
ACTCCGG9750.020.82038332
GAATCGG7400.020.81132740
TCCGGAG10100.020.09950834
CACTCCG10500.019.00047331
CCGGAGA10800.018.4726835
CGGAGAA11250.018.04489136
CTCCGGA11600.017.50043533
GTCACTC12850.016.06993929
AGAATCG10100.015.59444639
TCACTCC14200.014.54261530
CAGTCAC14200.014.54216427
CTGGACG1500.00593440414.00078246
AGTCACT15150.013.63027828
GGAGAAT14450.013.32213137
CCAGTCA15750.012.88880826
Produced by FastQC (version 0.11.5)