FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_pIXHALO_F1a_B_h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_pIXHALO_F1a_B_h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences593771
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG15510.2612118139821581TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGGG3650.045.07039343
AATCGGG4150.044.70067641
ATCGGGG3800.044.21238742
CGGGGGG3750.040.1350244
GAATCGG4800.037.91826240
ACTCCGG6150.037.56255732
CACTCCG6150.036.42429731
TCCGGAG6550.034.73721334
CTCCGGA6550.034.73428733
CCGGAGA6450.034.7330735
AGAATCG5550.034.05548539
CGGAGAA6900.032.97240436
CGGGGGT652.1017127E-532.3090544
CCAGTCA7050.031.77438726
GGAGAAT6900.030.94333337
TCACTCC7250.030.8978530
GTCACTC7350.030.47747229
CAGTCAC7450.030.06837827
TCCAGTC7900.028.35323725
AGTCACT8200.027.31822228
Produced by FastQC (version 0.11.5)