FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_VvMYB_D2_Vv_B_h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_VvMYB_D2_Vv_B_h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43698066
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGCG105900.025.8144841
ATCGCGT39650.024.1006542
CATCGGG371800.024.02589641
CGCATCG514450.023.81352839
ATCGCGG74400.023.80602542
GCATCGG393800.023.41186140
GCGCATC632250.023.30728338
ATCGGGG287850.023.12882442
AACCCCC328750.022.8930631
CGGCGTG35100.022.73465353
AACCACC601000.022.5756631
TGGCGGG115650.022.48543450
AACTACC304350.022.278581
GCATCGC131050.022.24866140
TGCGCAT725900.021.6985737
ACCTACC287250.021.4112951
GCGGCGT32400.021.2804552
GCGGGGT118550.021.2269252
CGGGGTG129950.021.19625353
TCGGGGT135800.020.4401143
Produced by FastQC (version 0.11.5)