FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_VvMYB_D1_Vv_B_h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_VvMYB_D1_Vv_B_h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32923850
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGCG55000.024.50197241
CATCGGG177250.023.1048241
CGCATCG247700.022.83542439
GCGCATC302950.022.37960638
ATCGGGG134150.022.23070542
GCATCGG188800.022.17275440
ATCGCGG40600.021.8983842
GCGGGGT59650.021.76845252
TGGCGGG58400.021.21520450
CGGGGTG64450.020.961753
TGCGCAT353250.020.62942137
ATCGCGT22200.020.49720842
GCATCGC72950.019.480140
CGGCGTG17650.019.4331653
ATGCGCA405000.018.97864236
TGCGGGG39150.018.23732651
GCGGCGT17450.017.8508452
TCGGGGT69350.017.61502543
AATGCGC454450.017.39114835
CGGGGTT65350.017.35411344
Produced by FastQC (version 0.11.5)