FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_VvMYB_A7_Vv_B_h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_VvMYB_A7_Vv_B_h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43767474
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGCG82200.023.88903441
CATCGGG289100.022.8713341
TGGCGGG91850.022.7863150
GCGGGGT93000.022.61754652
GCATCGG301250.022.5525240
CGCATCG398500.022.538639
ATCGGGG221900.022.27326842
GCGCATC490900.021.92553538
ATCGCGT33650.021.84443542
CGGGGTG103500.021.30377853
CGGCGTG29650.021.12928453
GCGGCGT27300.020.64035652
AACCACC294050.020.4878771
AACCCCC175850.020.483791
TGCGCAT574050.020.37142437
ATCGCGG59900.020.2188242
GCATCGC108950.019.40467340
ATGCGCA652750.018.93398536
TCGGGGT107900.018.75039743
CGGGGTT101650.018.35366844
Produced by FastQC (version 0.11.5)