FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_VvMYB_24_SD_Vv_B_h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_VvMYB_24_SD_Vv_B_h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25521749
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAAC391400.026.2491131
CATCGCG35250.023.03711541
ACCTAAT385950.021.8490771
CGCATCG159850.021.15255739
GCGCATC194350.021.089838
CATCGGG117400.020.15477241
TGCGCAT227900.019.67432437
ATCGCGT16650.019.5510742
GCATCGG126100.019.54118340
GCGGGGT41100.019.5008652
ATCGGGG93700.019.42520142
ATCGCGG26450.018.65929642
CGGGGTG47600.018.60252853
CCTAACA201750.018.5623862
ATGCGCA259150.018.17968236
GCATCGC48350.018.17065440
TGGCGGG45800.017.11759250
CCTAACT277150.016.909442
AATGCGC290500.016.65176835
CGGGGTT51100.016.16554644
Produced by FastQC (version 0.11.5)