FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_ColTGA5_Vv_B_h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_ColTGA5_Vv_B_h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54464540
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG328200.022.91910239
GCGCATC432150.020.95378538
TGCGCAT463750.020.92965937
CATCGGG267350.020.52891741
GCATCGG281250.020.34802240
ATGCGCA516450.019.93224336
CATCGCG89000.019.42840441
ATCGGGG214300.019.3062342
AATGCGC561400.018.92875135
ATCGCGG60250.018.88115342
GCGGGGT88250.018.63989452
TAATGCG604050.017.68493334
CGGGGTG100550.017.68254353
TGGCGGG97900.017.5888750
CGGGGGT89300.016.81523144
TCGGGGG103350.016.52768143
ATCGCGT35300.016.16274342
GCATCGC117750.015.695312540
CTAATGC712050.015.149837533
TGCGGGG56750.015.04813551
Produced by FastQC (version 0.11.5)