FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_ColTGA5_Col_UB_h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_ColTGA5_Col_UB_h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57642191
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG121500.040.24609842
ACTCCGG175650.034.1133432
CACTCCG175950.034.0552931
CGGGGGG125050.032.52540244
TCGGGGG148850.031.74591443
TCCGGAG225650.026.8350734
CTCCGGA234400.025.92256733
AATCGGG191650.025.67909641
GTCACTC243200.025.02669329
CAGTCAC246200.024.8068427
CCAGTCA260850.023.35997826
TCCAGTC274300.022.31613725
TCACTCC272200.022.30905230
CCGGAGA281200.021.32237235
GAATCGG236050.021.21942740
ACTCCAG289600.021.17343523
ACGTCTG296450.020.6966215
CTCCAGT299200.020.55252624
GCACACG300250.020.32888611
CGTCTGA305400.020.1130816
Produced by FastQC (version 0.11.5)