FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_ColTGA5_Col_B_h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_ColTGA5_Col_B_h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2768653
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG37360.13493926468936338TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG8300.042.59234242
CGGGGGG7900.039.4322144
TCGGGGG9000.037.33506843
AATCGGG11000.031.50146341
ACTCCGG15700.027.1972432
CACTCCG15700.026.97431231
TCCGGAG17150.024.89911534
CTCCGGA17100.024.56254633
CAGTCAC18700.023.39511727
CCAGTCA18900.022.7771926
GAATCGG15900.022.23373640
TCCAGTC19700.021.67456825
CGGGGGT1957.8034645E-1021.53946144
TCACTCC20300.021.37914530
AGAATCG17950.019.88878439
AGTCACT21700.019.83819828
ACTCCAG22300.019.30443423
CTCCAGT23050.018.67630624
GGAGAAT21750.018.66685937
GTCACTC24750.017.2520829
Produced by FastQC (version 0.11.5)