FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_ColTGA5_Col_18Y_B_h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_ColTGA5_Col_18Y_B_h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5620054
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTA1293200.069.6827556
ACGCACT1758600.069.634495
TCGCACT862900.069.530265
CGCACTG1197450.069.455986
CCGCACT675750.069.452875
CGCACTC815750.069.430376
AACGCAC784450.069.398844
GACGCAC396150.069.351774
CGCGCAC149900.069.296264
GGCGCAC112800.069.283074
CGCACTT593800.069.2776266
TACGCAC442350.069.26884
GCACTGT888200.069.23627
CACGCAC354650.069.11844
GCGCACT603050.069.097145
ATCGCAC372850.069.0861744
TTCGCAC254100.069.06014
GCACTAG320150.069.0456167
GCCGCAC140650.068.976484
GCACTAT333150.068.904147
Produced by FastQC (version 0.11.5)