FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_ColTGA5_Col_17Y_B_h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_ColTGA5_Col_17Y_B_h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10512837
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAACT4704400.068.896345
ACTGTAG917800.068.5278859
TAAACTG1951100.068.36796
GTAAACT1456500.068.237725
TAAACTA3528550.068.043676
TAAACTC2036050.067.9820566
CTGTAGA865800.067.9101710
TTAAACT2496900.067.787615
TAAACTT2499450.067.591036
AATAAAC2143250.067.192864
AAACTGT1166400.066.995797
AACTGTA988100.066.79768
AAACTAG858600.066.5763857
CTAAACT1332100.066.121375
CGTAAAC367550.065.6355444
TGTAGAA878900.065.6157511
GTAGAAG873650.065.6054512
TATAAAC1105850.065.569044
CATAAAC936750.065.550634
ATTAAAC1129350.065.475234
Produced by FastQC (version 0.11.5)