FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_ColTCP21_F1b_B_h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_ColTCP21_F1b_B_h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7499889
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAGTGCTGGTATGATCGCATCCGTTAGTATATGCAATGCAATCGTATA156580.20877642322439705No Hit
GTTCGCCGTGGGTCCCACCCGCCATGTAGGGATACCCCATCTAGTCTTAA153340.2044563592874508No Hit
GTTAAGACTAGATGGGGTATCCCTACATGGCGGGTGGGACCCACGGCGAA132230.17630927604395213No Hit
AAAGCTCGTTAAGACTAGATGGGGTATCCCTACATGGCGGGTGGGACCCA119420.15922902325621086No Hit
CATTAGTGCTGGTATGATCGCATCCGTTAGTATATGCAATGCAATCGTAT104590.139455397273213No Hit
CTTGATGAACCGTTCGCCGTGGGTCCCACCCGCCATGTAGGGATACCCCA101900.135868677523094No Hit
ATCAAAGCTCGTTAAGACTAGATGGGGTATCCCTACATGGCGGGTGGGAC98720.13162861477016527No Hit
GGTGCATTAGTGCTGGTATGATCGCATCCGTTAGTATATGCAATGCAATC97230.12964191870039676No Hit
GCATTAGTGCTGGTATGATCGCATCCGTTAGTATATGCAATGCAATCGTA95170.1268952113824618No Hit
GAGGACTTCCCGGGAGGTCACCCATCCTAGTACTACTCTCGCCCAAGCAC92990.12398850169649178No Hit
ATTGTTTTCGAATATTTTTCATGCATCAAAGCTCGTTAAGACTAGATGGG89640.11952176892218004No Hit
GTATATATTCTTTTTTTAAGTCTTGATGAACCGTTCGCCGTGGGTCCCAC88920.11856175471396976No Hit
TGGTATGATCGCATCCGTTAGTATATGCAATGCAATCGTATATATTCTTT88570.1180950811405342No Hit
ATATATTCTTTTTTTAAGTCTTGATGAACCGTTCGCCGTGGGTCCCACCC86430.11524170557724255No Hit
GAATATTTTTCATGCATCAAAGCTCGTTAAGACTAGATGGGGTATCCCTA85390.11385501838760546No Hit
TGATGAACCGTTCGCCGTGGGTCCCACCCGCCATGTAGGGATACCCCATC85340.11378835073425754No Hit
ATATGCAATGCAATCGTATATATTCTTTTTTTAAGTCTTGATGAACCGTT84980.1133083436301524No Hit
CATGCATCAAAGCTCGTTAAGACTAGATGGGGTATCCCTACATGGCGGGT84930.11324167597680446No Hit
GGTATGATCGCATCCGTTAGTATATGCAATGCAATCGTATATATTCTTTT83790.11172165348047151No Hit
AAGCATTGTTTTCGAATATTTTTCATGCATCAAAGCTCGTTAAGACTAGA82330.10977495800271178No Hit
GCCGTGGGTCCCACCCGCCATGTAGGGATACCCCATCTAGTCTTAACGAG82180.10957495504266797No Hit
CGTTAAGACTAGATGGGGTATCCCTACATGGCGGGTGGGACCCACGGCGA82080.10944161973597209No Hit
ACTAGATGGGGTATCCCTACATGGCGGGTGGGACCCACGGCGAACGGTTC77480.10330819562796197No Hit
ATATATTCTTTTTTGAAGACTTGATGAACCATTCGCCGTGGGTCCCACCC77470.10329486209729238No Hit
AGATGGGGTATCCCTACATGGCGGGTGGGACCCACGGCGAACGGTTCATC76290.10172150547828109No Hit
ATATGCAATGCAATCGTATATATTCTTTTTTGAAGACTTGATGAACCATT76050.10140150074221099No Hit
GTATATATTCTTTTTTGAAGACTTGATGAACCATTCGCCGTGGGTCCCAC75250.100334818288644No Hit
GTTAGTATATGCAATGCAATCGTATATATTCTTTTTTTAAGTCTTGATGA75080.10010814826726103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGG35350.025.44439769
TAACGGA35550.025.399770
GCCGTCT4400.024.65880251
ACTAACG36800.024.44183268
TGCCGTC5800.023.53389450
CGTATGC7550.022.25302746
GTATGCC7800.021.98809447
CGAAAAC53600.021.09046769
ACGGATG16350.020.76359270
TACTAAC49500.018.24172467
GAAAACA72150.017.4628370
CGGATGC11600.016.8957969
TATGCCG10500.016.33390448
TGCCGGC1503.1626565E-416.33292450
GGATGCG12250.015.71357770
TAGTCCG3009.771611E-915.16608411
CGCTCAT13850.015.16379434
TATACTA81950.014.819344570
TCGAAAA78500.014.5789668
GGACTGT5300.014.5277447
Produced by FastQC (version 0.11.5)