FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_ColTCP21_F1a_B_h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_ColTCP21_F1a_B_h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4475830
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGCCGTGGGTCCCACCCGCCATGTAGGGATACCCCATCTAGTCTTAA95230.21276500671383855No Hit
ATTAGTGCTGGTATGATCGCATCCGTTAGTATATGCAATGCAATCGTATA89780.20058849420107558No Hit
GTTAAGACTAGATGGGGTATCCCTACATGGCGGGTGGGACCCACGGCGAA86950.1942656445843564No Hit
AAAGCTCGTTAAGACTAGATGGGGTATCCCTACATGGCGGGTGGGACCCA75280.16819226824968778No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT65520.14638625685068468TruSeq Adapter, Index 2 (97% over 37bp)
CTTGATGAACCGTTCGCCGTGGGTCCCACCCGCCATGTAGGGATACCCCA64660.14446482551839546No Hit
ATCAAAGCTCGTTAAGACTAGATGGGGTATCCCTACATGGCGGGTGGGAC63260.14133691404722698No Hit
GGTGCATTAGTGCTGGTATGATCGCATCCGTTAGTATATGCAATGCAATC61290.13693549576279707No Hit
CATTAGTGCTGGTATGATCGCATCCGTTAGTATATGCAATGCAATCGTAT60860.13597478009665245No Hit
CATGCATCAAAGCTCGTTAAGACTAGATGGGGTATCCCTACATGGCGGGT59150.1321542596568681No Hit
TGATGAACCGTTCGCCGTGGGTCCCACCCGCCATGTAGGGATACCCCATC56650.12656870345835297No Hit
GCATTAGTGCTGGTATGATCGCATCCGTTAGTATATGCAATGCAATCGTA55430.12384295203347757No Hit
CGTTAAGACTAGATGGGGTATCCCTACATGGCGGGTGGGACCCACGGCGA54790.12241304964665772No Hit
ATTGTTTTCGAATATTTTTCATGCATCAAAGCTCGTTAAGACTAGATGGG53720.12002243159369323No Hit
ATGCATCAAAGCTCGTTAAGACTAGATGGGGTATCCCTACATGGCGGGTG53060.11854784475728525No Hit
GAATATTTTTCATGCATCAAAGCTCGTTAAGACTAGATGGGGTATCCCTA52840.11805631581181592No Hit
AGATGGGGTATCCCTACATGGCGGGTGGGACCCACGGCGAACGGTTCATC52230.11669344009937821No Hit
TGGTATGATCGCATCCGTTAGTATATGCAATGCAATCGTATATATTCTTT51660.11541993328611677No Hit
ACTAGATGGGGTATCCCTACATGGCGGGTGGGACCCACGGCGAACGGTTC50890.1136995819769741No Hit
AAGCATTGTTTTCGAATATTTTTCATGCATCAAAGCTCGTTAAGACTAGA50560.11296228855877011No Hit
AGTCTTGATGAACCGTTCGCCGTGGGTCCCACCCGCCATGTAGGGATACC48090.10744375903463715No Hit
AAAGCTCGTTAAGACTAGATGGGGTATCCCTACATAGCGGGTGGGACCCA47290.1056563810511123No Hit
GGTATGATCGCATCCGTTAGTATATGCAATGCAATCGTATATATTCTTTT46830.10462863871058552No Hit
ATCCCTACATGGCGGGTGGGACCCACGGCGAACGGTTCATCAAGACTTAA46650.10422647866429244No Hit
GAGGACTTCCCGGGAGGTCACCCATCCTAGTACTACTCTCGCCCAAGCAC46470.10382431861799934No Hit
CGTTCGCCGTGGGTCCCACCCGCCATGTAGGGATACCCCATCTAGTCTTA46310.10346684302129437No Hit
GCCGTGGGTCCCACCCGCCATGTAGGGATACCCCATCTAGTCTTAACGAG46240.10331044744773595No Hit
GTATATATTCTTTTTTGAAGACTTGATGAACCATTCGCCGTGGGTCCCAC45890.10252846957994383No Hit
CATCAAAGCTCGTTAAGACTAGATGGGGTATCCCTACATGGCGGGTGGGA45700.10210396730885667No Hit
GAACCGTTCGCCGTGGGTCCCACCCGCCATGTAGGGATACCCCATCTAGT45690.10208162508406261No Hit
GTTAAGACTAGATGGGGTATCCCTACATAGCGGGTGGGACCCACGGCGAA45290.1011879360923002No Hit
ATATGCAATGCAATCGTATATATTCTTTTTTGAAGACTTGATGAACCATT45160.10089748716997742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC9650.042.43427750
GCCGTCT8200.041.82832751
GTATGCC11550.041.82097647
CGTATGC11800.041.23249446
TATGCCG13150.036.73208648
CGCTCAT17700.034.60683434
ATGCCGT13500.034.22145549
CCGCTCA18400.033.28989833
TCGTATG15500.032.29356445
TATCTCG16050.031.62381741
TCTCGTA15150.031.19159743
ACCGCTC19500.031.05127532
TCACCGC19700.030.7353530
CTCGTAT15300.030.65667244
CCGTCTT11850.028.9448252
CACCGCT21200.028.56067831
GTCACCG21600.028.35552629
CTGCGTG2300.027.39129859
ATCTCGT18550.026.98414242
GCTCATT24350.025.73067535
Produced by FastQC (version 0.11.5)