FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_ColTCP21_Col_B_h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_ColTCP21_Col_B_h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25876861
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT395990.15302860729514295TruSeq Adapter, Index 2 (97% over 37bp)
GTTCGCCGTGGGTCCCACCCGCCATGTAGGGATACCCCATCTAGTCTTAA268700.10383794232229326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC55150.045.88249250
GCCGTCT44200.045.6094151
CGTATGC64700.044.90313746
GTATGCC66350.044.47117647
CGCTCAT98000.038.89484834
TATGCCG77650.037.68340348
CCGCTCA105350.036.48006433
ACCGCTC108650.035.24211532
ATGCCGT80350.034.323849
CTCGTAT90850.033.48133544
TATCTCG98950.033.35861641
TCGTATG93250.032.99460245
CACCGCT116750.032.85719731
CCGTCTT63900.032.2056152
TCACCGC120650.031.82385430
TCTCGTA96650.031.54490943
GTCACCG127900.029.88292329
ATCTCGT114800.028.63106542
CTAACGG106500.026.48690669
CAGTCAC145600.026.39456427
Produced by FastQC (version 0.11.5)