FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_ColTCP21_C24_B_h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_ColTCP21_C24_B_h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30066563
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT510220.1696968156952293TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT61150.042.0102851
TGCCGTC78050.041.52337350
CGGACTG33400.039.084966
CGTATGC99800.038.6148146
GTATGCC102200.038.1184247
TATGCCG109150.034.7930948
CCGTCTT82700.031.99448652
ATGCCGT112850.031.9441749
CGGACTA21950.031.41086
CTCGTAT128900.031.3640944
TCTCGTA132800.030.62781543
TCGTATG134450.029.93888345
GGACTGT39700.029.7970667
TATCTCG147550.029.74858541
ATCTCGT162500.026.75327142
CGCTCAT191100.026.44852634
CCGCTCA200700.025.23532533
GACTGTA43950.024.3673578
ACTGTAG61800.024.1817329
ACCGCTC209150.023.93012432
Produced by FastQC (version 0.11.5)