FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_ColSPL9_F1b_B_g-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_ColSPL9_F1b_B_g-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1015875
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT10630.10463885812723023TruSeq Adapter, Index 7 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2550.038.43123648
TGCCGTC2550.031.56851850
ATGCCGT3050.029.8359649
GTATGCC3200.029.53114547
GGGCATA657.9588615E-426.92298140
CGTATGC4200.024.16658246
TCGTATG4750.022.84202445
CTCGTAT4400.021.47719644
GCCGTCG1154.1190153E-521.30427451
GCCGTCT2650.021.13200251
AGCCGCT900.00527655819.44437637
CATCTCG4200.019.166641
CGGTCTC1000.00968981617.49993941
ATCTCGT5200.016.82686442
TCTCGTA5450.016.05498943
CCATCTC6150.013.65848940
TGGAGGC1550.007373813513.5483444
TCCATCT7300.012.94515939
GTCACGA8800.012.72722729
CCGTCTT4458.1854523E-1012.58422652
Produced by FastQC (version 0.11.5)