FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_ColSPL9_F1a_B_g-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_ColSPL9_F1a_B_g-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2970529
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT87020.2929444553478522TruSeq Adapter, Index 7 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC14900.047.9230247
TGCCGTC12200.047.90936350
TATGCCG15150.046.43831648
GCCGTCT10200.045.9799451
ATGCCGT15500.042.22681449
CGTATGC18450.039.27319346
CTCGTAT19450.036.53412244
TCGTATG20900.035.5068245
CATCTCG18850.034.9109341
TCTCGTA21550.030.37502343
CCGTCTT16050.030.31122652
ATCTCGT23900.028.12019242
CCATCTC24350.027.88802340
CACGAGA32500.027.78434231
GATTCCA33600.026.56581736
CGAGATT34450.026.41616833
TCACGAG34150.026.33897430
GTCACGA35200.025.75215329
TGCCGTG4200.024.99975450
AGATTCC36750.024.7641635
Produced by FastQC (version 0.11.5)