FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_ColSPL9_Col_B_h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_ColSPL9_Col_B_h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4215034
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT107750.2556325761547831TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG20400.046.8411548
ATGCCGT20050.046.08382849
TGCCGTC18350.045.58487750
GCCGTCT16100.044.12951751
CGTATGC22100.043.23798446
GTATGCC22950.042.0941247
CTCGTAT23600.037.67228344
TCGTATG25900.036.7594845
GATCTCG24050.033.4748141
CCGTCTT21850.033.31738352
TCTCGTA27950.030.05589343
TTACTCG36750.029.61983735
CGATCTC27400.029.25329840
CTCGATC31850.027.25444238
TACTCGA39450.026.52827336
ACTCGAT36700.026.41791237
ATCTCGT31300.025.7210642
CTGCTTG18150.025.64745759
CAGTCAC43450.025.05126227
CCAGTCA45450.023.87217126
Produced by FastQC (version 0.11.5)