FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_ColSPL9_C24_B_h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_ColSPL9_C24_B_h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5614253
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT16100.029.347449
TATGCCG16650.029.21931548
TGCCGTC14800.028.14123250
GCCGTCT12350.028.05602351
CGTATGC17100.027.8375946
GTATGCC17850.026.86307147
CGGACTG5950.023.5286526
CTCGTAT19550.022.20094544
TCGTATG21250.021.90673445
CCGTCTT19150.019.19049352
ACTGTAG13150.018.6305779
GATCTCG24900.016.72800641
TCTCGTA24350.016.67475343
CGATCTC25900.015.81199140
CTGCGTG4550.015.38411859
TTACTCG36200.015.37434935
GGACTGT9650.014.5073037
ATCTCGT29450.014.50010442
CAGTCCG5900.013.05077427
TACTCGA41150.012.92904436
Produced by FastQC (version 0.11.5)