FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_ColSPL9_2019poolJ_B_h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_ColSPL9_2019poolJ_B_h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2255174
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGGCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAG38260.1696543149220415TruSeq Adapter, Index 6 (97% over 36bp)
GGCGCCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAG28750.12748461981204112TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTCA10950.067.120335
GCCGCTA26250.066.930384
GCGCTAG21750.066.617755
CGCTAGA49450.066.31666
CGGCGCT15450.066.145953
CCGCTAG21800.066.1438755
GAGCGCT17950.065.902393
GGGCGCT18400.065.622113
GGCGCTA30200.065.477254
GCGCTAT21200.065.044315
GGAGCGC8350.064.9686362
ACGCTAG9700.064.9455955
GGCGCTC11500.064.8232354
CAGCGCT9000.064.552713
AGCGCTC12900.064.028194
AGGGCGC5550.063.6923032
GGCCGCT12350.063.4789853
GCGCTCT14250.062.874425
AGCGCTA19000.062.628824
GCGCGCT10350.062.5576323
Produced by FastQC (version 0.11.5)