FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_ColSPL9_2019poolI_B_h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_ColSPL9_2019poolI_B_h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1951011
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGGCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAG41340.21189014311041812TruSeq Adapter, Index 6 (97% over 36bp)
GGCGCCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAG33250.17042446198406877TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAG22500.068.285015
CGCTAGA54800.067.441436
GCGCTAT19550.066.952725
GAGCGCT19900.066.478643
AGCGCTC14550.066.147454
GCCGCTA26200.066.122214
CAGCGCT8100.066.107363
GGCGCTC9650.066.006624
GGGCGCT18000.065.9129263
GGCGCTA28650.065.720534
AGCGCTA17000.065.672754
CCGCTAG23900.065.6029745
GGCCGCT9300.065.1038363
GCCCGCT5300.064.713313
CGCCGCT15050.064.64753
GCGCGCT9850.064.311073
GGAGCGC8250.064.060262
GCGCTCA10600.064.052975
AAGCGCT11600.063.9618873
AGGGCGC5750.063.912692
Produced by FastQC (version 0.11.5)