FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_ColMYB113_Vv_B_h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_ColMYB113_Vv_B_h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22703775
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGGG92700.022.24022141
CGCATCG122000.022.20663639
GCGGCGT11250.022.08826352
GCATCGG98700.021.4200540
GCGCATC148850.021.11685238
ATCGGGG74750.020.88460542
TGGCGGG31100.020.819350
CATCGCG26500.020.34128641
CGGCGTG11600.019.91340453
GCGGGGT32400.019.1198352
TGCGCAT176450.018.64674837
ATCGCGT10800.017.50139442
CGGGGTG35750.017.42623353
ATGCGCA200850.017.30487836
CGGGGTT39950.016.0335744
ATCGCGG22600.015.95259842
AATGCGC227450.015.72764635
CGGGGGT34400.015.26260944
TCGGGGT43150.015.08807543
GCATCGC39300.014.60669640
Produced by FastQC (version 0.11.5)