FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_ColLHY1_F1b_B_g-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_ColLHY1_F1b_B_g-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704372
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG753.5377525E-837.3346641
CGTATCG1251.05501385E-1030.80328239
ATCGCGT500.007204758528.00298142
TGCGAGG500.007209754527.99900617
GTATCGC1056.768296E-726.66761440
TCGTATC3050.025.2485938
ATGCCGT700.001226336824.99911349
TATGCCG750.001831950923.33416248
GTATGCC850.0037885520.58896647
ATTCGTA4650.018.81787336
CACGAAT5150.018.35016631
CTGTCCA1000.00968478117.5006230
AATTCGT5650.016.72744635
TTCGTAT4850.016.59852637
CGAATTC6150.015.93665533
CGTATCT2452.4709516E-715.71595939
GTCACGA6150.015.36639929
ACGAATT7000.015.00053332
TCACGAA7250.014.48327330
TGGGTTC2001.7516817E-414.00049627
Produced by FastQC (version 0.11.5)