FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_ColLHY1_F1a_B_g-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_ColLHY1_F1a_B_g-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1403703
Sequences flagged as poor quality0
Sequence length76
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1950.041.28278448
ATGCCGG953.6379788E-1240.52559749
CGTATCG3150.040.00356339
TCGTATC9300.036.1322538
TATCGCG1850.035.9491541
GTATCGC1950.035.89935340
TGCCGGC751.4923553E-632.66608450
ATGCCGT1750.031.99942849
GCGGGGT1450.031.3787552
CGTATGC2350.031.27826746
CGGGGTG1357.2759576E-1231.11055653
CGTATCT7050.029.78988839
GTCACGA13200.029.6964429
CACGAAT13550.028.93040531
GTATGCC3050.028.68903547
AGTCACG13950.028.09985728
TGCCGTC1253.5634002E-927.99950250
CGAATTC14250.027.01898633
GGCGGGG1455.9480953E-1026.5512551
CGCGTAT1207.4058335E-826.25233844
Produced by FastQC (version 0.11.5)