FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_ColCAMTA1_F1b_B_g-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_ColCAMTA1_F1b_B_g-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1563892
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTG1600.030.62403553
TATGCCG2050.029.02718748
AATCGCG2500.026.60256441
GTATGCC2550.026.08094647
ATGCCGT1659.640644E-1125.45455749
GTCCCGC1101.017861E-625.4529365
GCGGGGT1951.8189894E-1225.12821652
ATCGCGG1202.168561E-623.33483542
CGCTGGC750.001833319923.33334530
GTGGGGG3300.023.33259857
TGCCGTG2004.3655746E-1122.75001150
CGTATGC2500.022.40215946
CATTCAG17700.022.3439333
GCGGCGT952.78202E-422.10527252
CCGTGGT1604.913818E-821.8750152
CGCGCCA800.002668556321.87361111
TGGGGGT3550.020.70357158
CCGGGGT850.003791357220.58824352
CGGCGTG1055.468931E-419.99936953
TGCCGGG1257.828638E-519.6000150
Produced by FastQC (version 0.11.5)