FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_ColCAMTA1_F1a_B_g-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_ColCAMTA1_F1a_B_g-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510249
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT5980.11719768191608411TruSeq Adapter, Index 13 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGCG200.006947755352.50396734
ACGCGGG200.006947755352.50396735
GCGCGAC200.00695044952.4988218
TGCGCGA200.00695044952.498828
GTGGGGG1700.045.29310257
CGGGGTG1500.044.3323453
GCGGGGT1350.044.07308652
GTATGCC1150.042.61191647
TATGCCG1250.042.00317848
GGCGGGG855.638867E-1141.1755551
GGGTGTA953.6379788E-1240.5254161
ACCGCGC350.001255251139.99910426
TGCGGGG1050.039.99910451
AATCGCG1800.038.8918341
ATGCCGT1200.037.91581749
TATGGCG753.533205E-837.33615548
TGCCGTC753.5362973E-837.33249750
CATTCAG9700.037.16775533
CGTATGC1051.4551915E-1136.6694446
CGTGGTA400.002418442634.99921453
Produced by FastQC (version 0.11.5)