FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_ColCAMTA1_Col_B_h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_ColCAMTA1_Col_B_h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5946102
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT90360.1519651025159003TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG18600.042.71726248
TGCCGTC16450.041.48836550
ATGCCGT19200.040.10320349
GTATGCC20750.039.13488447
GCCGTCT14850.038.65194351
CGTATGC21200.038.14004546
CTCGTAT24700.031.17674844
TCGTATG26900.030.96918545
CCGTCTT21250.027.66992252
GATCTCG26650.026.66335941
CGATCTC27450.026.65094840
TTACTCG38600.024.8466835
TCTCGTA30700.023.82959443
CAGTCAC41100.023.41851827
TACTCGA41150.023.0515936
TCCAGTC42950.022.49091725
CCAGTCA42750.022.43296626
CTCGATC34600.022.05390538
ATCTCGT34150.021.31989342
ACTCGAT40400.021.31387937
Produced by FastQC (version 0.11.5)