FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_ColCAMTA1_C24_B_h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_ColCAMTA1_C24_B_h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19935396
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT321520.16128096978861117TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG89050.033.5274848
GCCGTCT68300.032.89809451
ATGCCGT88750.032.84990749
TGCCGTC82500.032.62328350
GTATGCC95650.031.61703147
CGTATGC99250.030.57655546
CGGACTG31550.028.2876726
CTCGTAT105550.026.03229944
TCGTATG117700.025.39700345
CCGTCTT92900.024.52571752
GGACTGT38300.021.5659477
GATCTCG123600.021.1261541
TCTCGTA126100.020.8741243
CGATCTC133200.019.44567740
ATCTCGT140700.018.73295642
TTACTCG184650.018.46331235
CGGACTA23350.018.286456
ACTGTAG52450.018.0832239
TACTCGA181950.017.89075736
CTGCTTG75150.017.51117359
Produced by FastQC (version 0.11.5)