FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_C24SPL9_F1b_B_g-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_C24SPL9_F1b_B_g-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1328526
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT20850.15694085023552418TruSeq Adapter, Index 7 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3600.038.88803550
GCCGTCT3200.032.81177551
ATGCCGT3800.032.2361349
TATGCCG4450.031.46116648
AGCGGGC450.004309959231.10925758
CGGTCCC604.985348E-429.16602565
GCCCCCT500.00720889428.00149238
CGTATGC4500.028.00149246
GGGCCCG500.00721286927.9983359
GTATGCC4900.027.85862547
TCGTATG5900.023.73007645
CCGTCTT4800.022.60366852
GCCGTCG1553.5106495E-822.58014951
CTCGTAT6250.021.84198444
CTGCGTG1454.6123932E-721.72284159
CATCTCG6600.021.21404841
GAGGGCC900.005276812219.44474831
ATCTCGT6900.018.77052742
GACGTAC950.00721209818.4206454
TCTCGTA7250.017.3808943
Produced by FastQC (version 0.11.5)