FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_C24SPL9_F1a_B_g-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_C24SPL9_F1a_B_g-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387727
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT18710.48255602524456637TruSeq Adapter, Index 7 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC3000.054.83323346
ATGCCGT2750.054.7271749
GTATGCC3050.053.93432647
GCCGTCT1950.053.84605851
TATGCCG3000.053.6665748
CGGGTAG200.00694863152.4999055
TGCCGTC2400.052.49990550
TGCCGGC200.00694863152.49990550
TCGTATG3500.049.99991245
GCCGTGT651.8553692E-1048.46145251
CCGTCTT2200.047.72718452
CCGGCAC305.8746117E-446.6665813
ATCGCTC305.8746117E-446.6665843
CTCGTAT3450.044.63759644
ATCTCGT3600.041.80547742
TCTCGTA3550.041.40837543
CATCTCG3700.040.67560241
TCGCCCT350.001254751139.99992830
AGCGTCG350.001254751139.99992851
GCCTGCT350.001254751139.99992850
Produced by FastQC (version 0.11.5)