FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_C24SPL9_Col_B_h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_C24SPL9_Col_B_h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3110506
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT74870.24070038765397012TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG11950.047.4508648
GCCGTCT9150.046.66602351
CGTATGC13450.044.24157746
GTATGCC13300.044.2134747
ATGCCGT13300.043.15660549
TGCCGTC11650.042.9607850
TCGTATG16750.036.5701945
CTCGTAT16100.035.65512544
GATCTCG16750.033.85341
CGATCTC18200.031.73237440
TCTCGTA17650.031.53235443
CCGTCTT14100.030.77971852
TTACTCG23800.029.56079135
CTCGATC21700.027.4211838
ATCTCGT21400.026.66108142
CAGTCAC27050.026.65397627
ACTCGAT25550.026.0291337
TACTCGA27450.025.24758336
GTCACAT29500.025.03355229
CCAGTCA28850.024.62704526
Produced by FastQC (version 0.11.5)