FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_C24SPL9_C24_B_h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_C24SPL9_C24_B_h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2953681
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7050.031.77443948
TGCCGTC6200.031.61214650
CGGACTG3650.029.7248136
ATGCCGT7650.029.28034449
GCCGTCT5550.029.00831451
CGTATGC9050.025.52641346
GTATGCC9000.025.27888547
CGGACTA3200.021.8741076
CCGTCTT8700.019.30988352
TCGTATG12650.018.26197845
ACTGTAG9100.017.6915869
GGACTGT5800.017.4992857
CTCGTAT11650.017.42595144
GCGGACT2653.502646E-815.848415
GATCTCG12350.015.58772841
TTACTCG18000.015.55597435
CTGTAGA10800.015.2308610
ATAAACT40500.014.6907585
CGATCTC14200.014.5428940
CAGTCCG2656.0375896E-714.52844627
Produced by FastQC (version 0.11.5)