FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_C24CAMTA1_F1a_B_g-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_C24CAMTA1_F1a_B_g-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207078
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT2490.12024454553356706TruSeq Adapter, Index 13 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACCC150.002222899669.9954161
CGGGGTC252.3872186E-455.9963353
ACCCTGG200.006939198452.5092429
CGGACGG200.006939198452.5092423
CGTACTA200.00694583152.49655560
GGTTCGC200.00694583152.49655542
TATGGCG305.870076E-446.6636148
GCGGGGT757.512426E-1041.99724652
CGGGGTG602.587385E-740.83065853
CCGTCCT350.001252301340.0070436
TCCCTGT350.0012537939.9973853
TATGCCG350.0012537939.9973848
ACCCGGT400.00241278635.0061634
GCTCCAG501.7063109E-434.99770744
TTCGGCA400.002415647334.9977044
ATCGCGG400.002415647334.99770442
TGCCGTG400.002415647334.99770450
GGAGTTC751.4847847E-632.6645245
AATCGCG751.4847847E-632.66452441
GTATGCC552.9861933E-431.81609547
Produced by FastQC (version 0.11.5)