FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_C24CAMTA1_Col_B_h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_C24CAMTA1_Col_B_h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4775398
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT100300.2100348494512918TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG17950.046.9927648
TGCCGTC16250.046.30685450
ATGCCGT18150.045.31598349
GCCGTCT14150.044.0279351
GTATGCC20100.043.0114247
CGTATGC20250.042.5213146
CTCGTAT23150.033.86902644
TCGTATG26200.033.53308545
CCGTCTT21500.030.27915852
TTACTCG35250.030.18511635
GATCTCG24850.029.72119541
CAGTCAC39200.027.2316527
TCTCGTA27400.027.21038643
CGATCTC27300.027.18098640
TACTCGA38300.026.50249136
CCAGTCA41400.025.44666526
ATCTCGT29500.025.15498742
TCCAGTC43850.024.34391625
CTCGATC33550.023.68213338
GTCACAT45700.023.51160829
Produced by FastQC (version 0.11.5)