FastQCFastQC Report
Wed 8 May 2019
HCH2WBGXB_n01_C24CAMTA1_C24_B_h.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCH2WBGXB_n01_C24CAMTA1_C24_B_h.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5036268
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT10500.026.3320351
TATGCCG15350.026.22280348
TGCCGTC13000.025.5759150
CGTATGC15750.025.55733546
ATGCCGT15450.024.91835449
GTATGCC16850.024.30412347
CGGACTG6550.022.9768736
GGCGCTA2501.8189894E-1220.9995864
CTCGTAT19050.019.47642144
ACTGTAG13100.017.900419
TCGTATG21550.017.86667445
CCGTCTT16050.017.44479252
GGACTGT9850.015.2790387
TTACTCG33050.014.93251835
TCGGACT7600.014.7365515
TCTCGTA22950.014.64154343
GCGCTAG1951.4160882E-414.358695
GATCTCG21950.014.35208141
ATCTCGT24700.012.89589142
CGATCTC25250.012.61448940
Produced by FastQC (version 0.11.5)