FastQCFastQC Report
Wed 8 May 2019
HCGVJBGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGVJBGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40959976
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG769797718.79390017220713No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA1440300.3516359482241884No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC941870.22994886520441318No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA875870.21383557451303195No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT718520.1754200246601707No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAACTGTAGGC706590.17250742529731952No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA689980.16845224713998858No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA665550.16248788817649698No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG630140.15384286357980287No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA486880.11886725714878349No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG462490.11291266381601396No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGGT549950.069.150267
CTATCGG345300.068.9508921
GCTATCG346900.068.9010120
TAGTAGG550050.068.8801356
TGTATAG727400.068.8310114
GTAAGGT352750.068.800392
GTAGTAG1020600.068.791735
GGTAGTA1027950.068.769514
GTTGTAT564950.068.7452912
GAGGTAG1124800.068.7112
TAGGTTG551600.068.7022559
TGAGGTA1068850.068.700571
GGTCAGC350800.068.521686
TATAGTT638450.068.2689816
GTCAGCT352100.068.136377
CAGACTG679400.068.0437249
TTGTATA738200.067.96142613
AGGTAGT1036550.067.892563
GACTGAT688650.067.7093511
AGCTATC354550.067.69559519
Produced by FastQC (version 0.11.5)