FastQCFastQC Report
Wed 8 May 2019
HCGVJBGXB_n01_AL242.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGVJBGXB_n01_AL242.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6284633
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1020551.623881617271844No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG603210.9598173831312028No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA600790.9559667207297546No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC439230.6988952258628308No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC421240.6702698471016525No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT380480.6054132357450307No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG372200.5922382420739604No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA345410.5496104545802436No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT297550.47345644526896No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA279230.44430597618031153No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC262350.41744681033880576No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC236390.37613970457781704No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT235100.3740870787522517No Hit
TCAAATGATGAAATCACCCAAAATAGCTGGAATTACCGGCAGATTGTGTA232840.37049100560048615No Hit
CGTCCATGATGTTCCGCAACTACCTACATTGTTTGATCCTCATGAAAGCA230450.3666880786833535No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTA229100.3645399818891572No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA222850.35459508932343387No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG200390.3188571233992502No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT198550.31592934702790126No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCTA198270.3154838158409568No Hit
GTAAATGATGACTTCACTTTTTTCCCCATCAGATCGACAATGCTGACGTC196140.3120945964545583No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC195590.3112194459087746No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG194700.3098032932074156No Hit
CAAAATGATGAGATTCCACTTAATTGGTCCGTGTTTCTGAAACACATGAT194130.30889631900542164No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG181870.2893884177484986No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG179370.2854104607222092No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC174990.2784410800121503No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT173630.27627707138984886No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA169810.2701987530536787No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG157580.2507385872810711No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT151520.24109601944934572No Hit
TGCAGATGATGTAAAAGAATATTTGCTATCTGAGAGATGGTGATGACATT147880.23530411401906842No Hit
CCACAATGATGACAGTTTATTTGCTACTCTTGAGTGCTAGAATGATGAGG140120.22295653540946622No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT138250.21998102355380178No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTC135320.21531885791899064No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGG134580.214141382639209No Hit
GCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACAC129010.20527849438463627No Hit
AACCAATGATGTAATGATTCTGCCAAATGAAATATAATGATATCACTGTA117830.18748907056307026No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG115640.1840043802080408No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG109220.1737889865645297No Hit
CACCAGTGATGAGTTGAATACCGCCCCAGTCTGATCAATGTGTGACTGAA107310.17074982739644465No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGT101970.16225291118829055No Hit
TGTTATGATGAGATTCCACTTAAGGTCCGTGTTTCTGAAACAAATGATTT98870.15732024447569173No Hit
GCATATGATGGAAAAGTTTTAATCTCCTGACACTTGTGATGTCTTCAAAG98270.15636553478938228No Hit
TGCATATGATGGAAAAGTTTTAATCTCCTGACACTTGTGATGTCTTCAAA97800.15561767886843988No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTC95990.15273763798140638No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA95490.15194204657614852No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACT94190.14987350892247805No Hit
GTTAGTGATGATTTTAAAATTAAAGCAGATGGGAATCTCTCTGAGAAAGA89440.14231539057252826No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA88260.14043779485611968No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTGAACTTTCAAGTGA87010.13844881634297498No Hit
TCTCGTGATGAAAACTCTGTCCAGTTCTGCTACTGAAGGGAGAGAGATGA86430.13752593031287585No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAA84210.13399350447353092No Hit
TTGCAATGATGTCGTAATTTGCGTCTTACTCTGTTCTCAGCGACAGTTGC80510.12810612807462265No Hit
ATAAGTGATGAAAAAAGTTTCGGTCCCAGATGATGGCCAGTGATAACAAC78850.12546476460916653No Hit
TCAGATGATGAATTTAACTGTTCAACTGCTGAATGATAACGGGCATGAAC78850.12546476460916653No Hit
CTCACTGATGAGTACGTTCTGACTTTCGTTCTTCTGAGTTTGCTGAAGCC78110.12428728932938488No Hit
GTACATGATGACAACTGGCTCCCTCTACTGAACTGCCATGAGGAAACTGC77580.12344396243981151No Hit
TTTGAATGATGACTTTAATTGTCGGATACCCCTTCACTCCTTTTATGAGT77410.12317346136202384No Hit
AATCAATGATGAAACCTATCCCGAAGCTGATAACCTGAAGAAAAATAAGT74290.11820897099321473No Hit
TGTAATGATGTTGATCAAATGTCTGACCTGAAATGAGCATGTAGACAAAG69730.11095317737726293No Hit
TTGCAATGATGTGAATCTCTCACTGAATTCAACCTTGAAGTGCGAATCCA68560.1090914934889595No Hit
TTGAATGATGACTTTAATTGTCGGATACCCCTTCACTCCTTTTATGAGTG68260.10861413864580477No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATAACTATGAAAGAAAA68240.10858231498959448No Hit
GCAAATGATGCATATGTTAGCGACCAAAGCCTGATCTTTGCTGATTAGTC68180.10848684402096351No Hit
TTCCTATGATGAGGACCTTTTCACAGACCTGTACTGAGCTCCGTGAGGAT66710.10614780528950536No Hit
AGCCTGTGATGCTTTAAGAGTAGTGGACAGAAGGGATTTCTGAAATTCTA65800.10469982893193605No Hit
GAGTTATGATGTGTGTAAATCCTATTCCATTGCTGAAATGCAGTGTGGAA64920.10329958805868218No Hit
CACAAATGATGAATAACAAAGGGACTTAATACTGAAACCTGATGTTACAT64760.10304499880899967No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA64030.10188343535732318No Hit
TCACAATGATGAATGGTCCAAAACATTCGCGGTTTCCACCAGAATTCAAG63200.10056275362459512No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGATGC55150.069.480495
GTAGGTT253850.069.3104258
AGTAGGT254200.069.283827
TAGGTTG254000.069.269499
AGCTTAT597350.069.256392
GTTGACA277200.069.20486518
GCTTATC597950.069.162933
ATTGGCG129200.069.14847616
TGTTGAC322900.069.1427917
CAGCACG136950.069.119334
CAGTGGC112350.069.115985
GTAGTAG460550.069.099115
ACAGTGG112200.069.083634
CTTATCA597900.069.0809254
TTGGCGA115300.069.077617
GGTAGTA463350.069.044074
TAGTAGG255150.069.028056
TGTATAG345100.068.96339414
GAGGTAG512500.068.88252
GTTGTAT265850.068.8555412
Produced by FastQC (version 0.11.5)