FastQCFastQC Report
Wed 8 May 2019
HCGVJBGXB_n01_AL241.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGVJBGXB_n01_AL241.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6034609
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC832751.3799568455885045No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA566790.9392323512592118No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG562560.9322227836136525No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC404580.6704328316880183No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC374460.6205207329919801No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT373020.6181344971977472No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA345540.5725971641244694No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG336830.5581637517857412No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA311770.516636620533327No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC272100.45089913861859154No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT260010.4308647005961779No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT240570.3986505173740337No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC211420.3503458136227219No Hit
CGTCCATGATGTTCCGCAACTACCTACATTGTTTGATCCTCATGAAAGCA209480.3471310237332692No Hit
TCAAATGATGAAATCACCCAAAATAGCTGGAATTACCGGCAGATTGTGTA206860.34278940027431765No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTA202660.3358295458744717No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA188540.3124311782254658No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG187670.31098949409978344No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCTA182390.3022399628542628No Hit
GTAAATGATGACTTCACTTTTTTCCCCATCAGATCGACAATGCTGACGTC176250.2920653185649642No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC175320.29052420794785544No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT175180.29029221280119394No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG174410.2890162394945555No Hit
CAAAATGATGAGATTCCACTTAATTGGTCCGTGTTTCTGAAACACATGAT173850.28808825890790934No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG171380.2839952016775238No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG166480.27587537154437014No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT159620.2645076093579551No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC156830.25988427750662885No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA140530.2328734140024648No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT139350.23091802633774614No Hit
TGCAGATGATGTAAAAGAATATTTGCTATCTGAGAGATGGTGATGACATT137750.22826665323304293No Hit
GCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACAC136250.22578099094738366No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTC130910.21693203321043666No Hit
CCACAATGATGACAGTTTATTTGCTACTCTTGAGTGCTAGAATGATGAGG127900.21194413755721372No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT123340.20438772420880957No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG121060.20060951753460748No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGG111760.18519841136352No Hit
AACCAATGATGTAATGATTCTGCCAAATGAAATATAATGATATCACTGTA110590.18325959478070578No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG102110.16920731732577868No Hit
CACCAGTGATGAGTTGAATACCGCCCCAGTCTGATCAATGTGTGACTGAA100820.1670696477601117No Hit
TGCATATGATGGAAAAGTTTTAATCTCCTGACACTTGTGATGTCTTCAAA98300.16289373512020414No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACT97770.1620154677792712No Hit
GCATATGATGGAAAAGTTTTAATCTCCTGACACTTGTGATGTCTTCAAAG97370.1613526245030954No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGT93580.15507218446132964No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA92550.15336536302517695No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG90420.14983572257954078No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTC84060.13929651448834549No Hit
TGTTATGATGAGATTCCACTTAAGGTCCGTGTTTCTGAAACAAATGATTT84040.1392633723245367No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAA82220.13624743541793677No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTGAACTTTCAAGTGA82050.13596572702556206No Hit
GTTAGTGATGATTTTAAAATTAAAGCAGATGGGAATCTCTCTGAGAAAGA81330.1347726091284456No Hit
TCTCGTGATGAAAACTCTGTCCAGTTCTGCTACTGAAGGGAGAGAGATGA78200.12958586049236992No Hit
CTCACTGATGAGTACGTTCTGACTTTCGTTCTTCTGAGTTTGCTGAAGCC76320.12647049709434366No Hit
TCAGATGATGAATTTAACTGTTCAACTGCTGAATGATAACGGGCATGAAC74920.12415054562772833No Hit
ATAAGTGATGAAAAAAGTTTCGGTCCCAGATGATGGCCAGTGATAACAAC72400.11997463298782074No Hit
TTTGAATGATGACTTTAATTGTCGGATACCCCTTCACTCCTTTTATGAGT71540.11854951994404277No Hit
GAGTTATGATGTGTGTAAATCCTATTCCATTGCTGAAATGCAGTGTGGAA70790.11730668880121314No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATAACTATGAAAGAAAA70140.11622956847742744No Hit
TTGCAATGATGTCGTAATTTGCGTCTTACTCTGTTCTCAGCGACAGTTGC70050.1160804287402879No Hit
AATCAATGATGAAACCTATCCCGAAGCTGATAACCTGAAGAAAAATAAGT69600.11533473005459012No Hit
GTACATGATGACAACTGGCTCCCTCTACTGAACTGCCATGAGGAAACTGC68940.11424103864890002No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA68750.11392618809271654No Hit
TGTAATGATGTTGATCAAATGTCTGACCTGAAATGAGCATGTAGACAAAG68300.11318048940701873No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA65570.10865658404711888No Hit
TTCCTATGATGAGGACCTTTTCACAGACCTGTACTGAGCTCCGTGAGGAT64090.1062040639252684No Hit
TTGCAATGATGTGAATCTCTCACTGAATTCAACCTTGAAGTGCGAATCCA64000.10605492418812884No Hit
GCAAATGATGCATATGTTAGCGACCAAAGCCTGATCTTTGCTGATTAGTC62740.10396696786817505No Hit
CACAAATGATGAATAACAAAGGGACTTAATACTGAAACCTGATGTTACAT61990.10272413672534542No Hit
TTGAATGATGACTTTAATTGTCGGATACCCCTTCACTCCTTTTATGAGTG61300.10158073207394216No Hit
CCACAATGATGGCAATATTTTTCGTCAACAGCAGTTCACCTAGTGAGTGT60800.10075217797872241No Hit
AGCCTGTGATGCTTTAAGAGTAGTGGACAGAAGGGATTTCTGAAATTCTA60500.10025504552159054No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT252250.069.5344548
AAGATGC51800.069.384815
AGTAGGT253100.069.383897
GATAGGC27450.069.3559715
GTTGACA270650.069.3480518
AGCTTAT582200.069.332222
GCTTATC581900.069.325863
TAGGTTG252700.069.3136759
CAGCACG141300.069.27464
TGTATAG345050.069.1718914
TAGTAGG253400.069.1504066
AGATGCT51900.069.116266
CTTATCA583550.069.0932854
TGTTGAC317000.069.0660817
GTAGTAG459250.069.0098345
CAGACTG585050.068.885669
GGTAGTA463050.068.851634
GTTGTAT268400.068.8329712
GAGGTAG515200.068.8046952
TAGTTTG119650.068.793089
Produced by FastQC (version 0.11.5)