FastQCFastQC Report
Wed 8 May 2019
HCGVJBGXB_n01_AL240.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGVJBGXB_n01_AL240.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6101127
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG322370.5283777898739036No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC257790.42252849350619975No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA247530.4057119283043936No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA165130.27065491342828957No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT148620.24359433920978862No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG146290.2397753726483648No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC121280.19878294616715894No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT118950.19496397960573514No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC113350.1857853475267766No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG106930.17526270146482772No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC101480.16632992560226986No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG95820.15705295103675107No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA93420.15311925157434028No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT91240.14954614122931714No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC90620.14852993553486102No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA89370.14648113373152205No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG76960.12614062942797288No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC63230.1036365904200978No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC62880.10306292591516289No Hit
CGTCCATGATGTTCCGCAACTACCTACATTGTTTGATCCTCATGAAAGCA61620.10099773369739722No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAT288400.069.070392
GTTGTAT147050.068.98910512
GCTTATC288850.068.986453
TAGGTTG148400.068.975199
GTAGGTT149150.068.839518
CAGCACG85050.068.757624
CTTATCA290200.068.532884
TGTATAG189700.068.5312814
AGTAGGT149850.068.517947
CAGACTG290300.068.47039
TAGTAGG149800.068.450176
GGTAGTA274250.068.371454
TGAGGTA292900.068.3639451
ACAGTGG62750.068.318934
GTTGACA84850.068.1761718
GGGCTAT21150.068.1685815
GACTGAT292850.068.1608811
TTGGCGA76400.068.15700517
AGGTTGT173450.068.13302610
GATGTTG292500.068.0624215
Produced by FastQC (version 0.11.5)