FastQCFastQC Report
Wed 8 May 2019
HCGVJBGXB_n01_AL239.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGVJBGXB_n01_AL239.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6312958
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG274740.4352001074615101No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC240190.38047140500538734No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA208570.3303839499645016No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA162520.25743874741444506No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG147940.23434339338230986No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT124150.19665899884016336No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT116030.18379656573035968No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC109280.17310427219696375No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC103470.16390097954081115No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA93520.14813974685084233No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC92890.14714179945439207No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG91220.1444964468320556No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG89730.14213622203727636No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT86690.1373207298385321No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA83220.1318240989406234No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC81320.12881441631640825No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG69530.11013854361141007No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT136250.069.139178
TAGGTTG135900.069.111239
GTTGTAT137050.069.0952412
AGCTTAT248850.068.905032
GCTTATC248900.068.8208853
AGTAGGT137200.068.6349267
CAGCACG76800.068.620874
TGAGGTA265300.068.477811
TAGTAGG138000.068.3643656
GGTAGTA249550.068.347154
CTTATCA250600.068.256274
ACAGTGG60400.068.249724
CAGACTG251000.068.244019
AGGTTGT159500.068.16627510
TGTATAG175800.068.07796514
GACTGAT253250.067.9571211
GTTGACA78600.067.8985418
CAGTGGC60650.067.852985
GATGTTG251800.067.8492215
CGTAAAT78300.067.841529
Produced by FastQC (version 0.11.5)