FastQCFastQC Report
Wed 8 May 2019
HCGVJBGXB_n01_AL238.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGVJBGXB_n01_AL238.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7419338
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA455820.614367481303588No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA343560.4630601813800639No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC250680.33787381030490865No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG248640.33512423884718556No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTAACTGTAGGCACCATCA178600.24072228546536092No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG174870.23569488275099476No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA156340.21071960867667708No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT150670.20307741741918214No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTAACTGTAGGCACCATC140710.18965303912559314No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT130400.1757569206309242No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT109470.14754685660634412No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC102390.13800422625307002No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG98930.13334073740810837No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA96860.13055073107600704No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACT94590.12749115891471718No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG94280.12707333187947495No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC88930.11986244594868167No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC88840.11974114132554682No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAACTGTAGGCACCATCA79140.10666719860990294No Hit
GGTTCGTGCTGAAGGCCTGTATCCTAGGCTACACACTGAGGAACTGTAGG77350.10425458443866556No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA75840.10221936242829212No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA74770.10077718524213347No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT160050.068.920188
TAGGTTG160250.068.834169
CAGCACG78300.068.782984
AGCTTAT200300.068.731772
GTTGTAT154950.068.6378612
AGTAGGT160950.068.6217657
TGAGGTA302450.068.5928961
GCTTATC201150.068.458733
TAGTAGG161600.068.409336
GGTAGTA288300.068.168954
CAGACTG202200.068.105079
CTTATCA202200.068.103234
TGTATAG195750.067.8100414
AGGTTGT185400.067.6703210
GTTGACA60350.067.6140818
CGTAAAT80350.067.596119
CTAGCGG54250.067.5416412
GTAAGGT48150.067.518622
GACTGAT205250.067.5036111
CAGTGGC63300.067.5019155
Produced by FastQC (version 0.11.5)