FastQCFastQC Report
Wed 8 May 2019
HCGVJBGXB_n01_AL237.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGVJBGXB_n01_AL237.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6210168
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA512610.8254366065459099No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA290970.4685380492121952No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG263350.4240626018490965No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC240300.3869460536333317No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA214810.3459004651726008No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT172840.27831775243439466No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTAACTGTAGGCACCATCA158540.2552910001790612No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT149800.24121730684258463No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG145840.23484066775649226No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC137110.22078307704397046No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT134170.21604890560126552No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC127270.20493809507246824No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG123630.19907673995292882No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG123400.19870637960196888No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACT122600.19741816968558662No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA120320.19374677142389707No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTAACTGTAGGCACCATC115340.1857276646944173No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC104200.16778934160879383No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA93480.1505273287292711No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC81680.13152623246263226No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG80940.13033463828997863No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA78230.1259708271982336No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAACTGTAGGCACCATCA77460.12473092515371564No Hit
CGTCCATGATGTTCCGCAACTACCTACATTGTTTGATCCTCATGAAAGCA72490.11672792104819064No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCTA72250.11634145807327596No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT71310.11482781142152676No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC71010.11434473270288341No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT67880.10930461140503767No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTA67050.10796809361679105No Hit
TCAAATGATGAAATCACCCAAAATAGCTGGAATTACCGGCAGATTGTGTA65450.10539167378402646No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA64620.10405515599577983No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG62910.10130160729951267No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT130350.069.103868
AGCTTAT220850.069.088282
TAGGTTG130550.068.944389
GCTTATC221450.068.932693
GTTGTAT132250.068.8795512
AGTAGGT131600.068.660217
GTTGACA68200.068.55472618
TGTATAG168900.068.5214914
GTACAGG20200.068.43062610
CAGCACG74600.068.3965154
GGTAGTA249050.068.38964
TGAGGTA265200.068.379971
CAGACTG223100.068.2957469
TAGTAGG132500.068.221356
CTTATCA223750.068.1302644
CATGGTC45450.067.988996
GTAGTAG250250.067.7819755
GTAAGGT39500.067.687982
CGTAAAT75800.067.6814359
CAGTGGC56500.067.513925
Produced by FastQC (version 0.11.5)