FastQCFastQC Report
Wed 8 May 2019
HCGVJBGXB_n01_AL236.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGVJBGXB_n01_AL236.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7562625
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA5305777.015778251599148No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA3256994.30669245136444No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAACTGTAGGC2546523.3672435166360883No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT2199422.9082758962661774No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC1419581.8770995520735194No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA1352221.788029949918183No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAACTGTAGGCA1147851.5177930943290194No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAACTGTAGG948481.254167699707443No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGA771621.0203071023619443No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGAACTG691480.9143386059734551No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA616780.8155633791177005No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC540640.7148840515032809No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAAACTGTAG488040.6453314821242624No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTAACTGTAGGCACCATCA472890.6252987553924729No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTAAC449130.593881092874498No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA378770.5008446141386093No Hit
GTTCTTGTAGTTGAAATACAACGATGGTTTTTCATATCATTGGTCGTGGT303440.4012363432009388No Hit
CAAATAAAGTATAGGCGATAGAAATTGAAACCAACTGTAGGCACCATCAA294310.3891638154741244No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGAA287100.3796300887588635No Hit
GAGAAAGCTCACAAGAACTGCTAACTCATGCCCCCATGTCTAACAACATG242280.3203649526454108No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAACTGTAGGCACCATC227010.30017355085039915No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAACTGTAGGCACCATCAAT222590.29432901935505196No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC200510.2651328077221864No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAACTGTAGGCACCATCAA197020.26051800796681046No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA181900.24052495000082644No Hit
TATAACTGAACTCCTCACACCCAATTGGACCAACTGTAGGCACCATCAAT179250.23702087568800517No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGAACTGTAGGCACCATCA178130.2355399084312656No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTAACTGTAGGCACCATC171220.22640286937405993No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC160700.21249235549825624No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT153920.20352721442620783No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA149040.19707442852184262No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG145170.19195715773293004No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAAAACTGTA138650.18333581263119617No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC138130.1826482206905671No Hit
CCAAATAAAGTATAGGCGATAGAAATTGAAACCAACTGTAGGCACCATCA135870.17965984033321764No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG132240.17485991967074926No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCAACTGTAGGCACCATCA131170.17344506702368556No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAACTGTAGGCACCATCA123810.16371299647939705No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC119080.15745855440405943No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTGAACTGTAGGCACCATC116330.15382225087188642No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG111370.14726368159203979No Hit
GAGCCCAGCGCCGAATCCCCGCCCCGCGGCGGGGCGCGGGACATGTGGCG107260.14182906067668305No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT107140.14167038561346096No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT104220.13780929240838996No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAACTGTAGGCACCAT103690.1371084775458257No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTTCAACTGTAGGCACCA101600.1343448868613742No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA100480.13286391960463464No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAACTGTAGGCACC99750.13189864630336687No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATA97030.12830201153699938No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG96850.12806399894216625No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAACTGTAGGCACCATCAAT88990.11767078230111899No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC86710.11465595609989918No Hit
GTTTCCGTAGTGTAGCGGTTATCACATTCGCCTAACTGTAGGCACCATCA86700.114642733177964No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTTAACTGTAGGCACCAT86620.11453694980248261No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGAACTGTAGGCACCATCAAT85400.1129237533263913No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAACTGTAGGCACCATCA84620.11189236541544768No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCAACTGTAGGCACCATCAAT84580.11183947372770699No Hit
CCCAAATAAAGTATAGGCGATAGAAATTGAAACCAACTGTAGGCACCATC81440.10768747624006214No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCAAACTGTAGGCACCATCAA81180.10734368026974761No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAAGG1101500.069.819361
GTAAGGT1106100.069.813882
GGTCAGC1105500.069.730576
GTCAGCT1105200.069.672577
AAGGTCA1107800.069.65894
GCTATCG1103000.069.6294220
CTATCGG1102450.069.60747521
AGGTCAG1108300.069.592745
TAAGGTC1109050.069.580863
CATGGGT924000.069.555512
GGTTATC566250.069.525717
CCGTAGT586400.069.5191355
TCAGCTA1107900.069.505948
TATCGGG1103700.069.5041922
GGCCCAT1071000.069.50159527
GTTTCCG583150.069.4938051
CGTAGTG587050.069.483446
AAGCTAT1108450.069.48051518
TGGGTGG925100.069.468564
ATGGGTG924900.069.4651263
Produced by FastQC (version 0.11.5)