FastQCFastQC Report
Wed 8 May 2019
HCGVJBGXB_n01_AL235.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGVJBGXB_n01_AL235.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5170123
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA3617166.996274556717509No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA2223864.301367685062812No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAACTGTAGGC1993403.8556142668172497No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT1381582.6722381653202447No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC1044472.020203387811083No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA908491.757192237012543No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAACTGTAGGCA753431.4572767417719075No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAACTGTAGG735211.4220358006956508No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGA501760.9704991544688589No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGAACTG476900.9224151920563592No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA426080.8241196582750545No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAAACTGTAG369750.7151667378126207No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC354120.6849353487334828No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTAACTGTAGGCACCATCA328840.6360390265376664No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTAAC289300.5595611555082926No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA264810.5121928433810955No Hit
CAAATAAAGTATAGGCGATAGAAATTGAAACCAACTGTAGGCACCATCAA204400.3953484278807293No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGAA187320.362312463359189No Hit
GTTCTTGTAGTTGAAATACAACGATGGTTTTTCATATCATTGGTCGTGGT187150.36198365106594177No Hit
GAGAAAGCTCACAAGAACTGCTAACTCATGCCCCCATGTCTAACAACATG179900.3479607738539296No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAACTGTAGGCACCATC153440.29678210750498585No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAACTGTAGGCACCATCAAT147840.2859506437274316No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC123970.23978152937560673No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTAACTGTAGGCACCATC119570.23127109355038553No Hit
TATAACTGAACTCCTCACACCCAATTGGACCAACTGTAGGCACCATCAAT116880.226068122557239No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAACTGTAGGCACCATCAA115250.2229153929219866No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC113930.22036226217442023No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGAACTGTAGGCACCATCA112930.21842807221414268No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG112120.21686137834631786No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA105310.2036895447168278No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAAAACTGTA103020.19926024970779227No Hit
CCAAATAAAGTATAGGCGATAGAAATTGAAACCAACTGTAGGCACCATCA96690.18701682725923544No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT96250.1861657836767133No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA93020.17991835010501683No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCAACTGTAGGCACCATCA88380.17094370868932907No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAACTGTAGGCACCAT84770.16396128293272713No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTGAACTGTAGGCACCATC80720.15612781359360312No Hit
GAGCCCAGCGCCGAATCCCCGCCCCGCGGCGGGGCGCGGGACATGTGGCG77930.15073142360442876No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG76210.1474046168727514No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC76170.14732724927434027No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAACTGTAGGCACCATCA75050.14516095651882943No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT73430.14202756878317982No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTTCAACTGTAGGCACCA72040.13933904473839404No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC71430.13815918886262474No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG67990.13150557539927No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAACTGTAGGCACC67950.1314282078008589No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCAAACTGTAGGCACCATCAA67150.12988085583263687No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG67010.129610069238198No Hit
GTTTCCGTAGTGTAGCGGTTATCACATTCGCCTAACTGTAGGCACCATCA65030.12578037311684848No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTTAACTGTAGGCACCAT62480.12084818871814075No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATA60780.11756006578566892No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCAACTGTAGGCACCATCAAT60760.11752138198646336No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT56390.10906897186005052No Hit
CCCAAATAAAGTATAGGCGATAGAAATTGAAACCAACTGTAGGCACCATC56060.10843068917315893No Hit
AGCCCAGCGCCGAATCCCCGCCCCGCGGCGGGGCGCGGGACATGTGGCGT52950.10241535839669577No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA52920.10235733269788747No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTTAACTGTAGGCACCATC52780.10208654610344861No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAACTGTAGGCACCATCAAT52590.10171905001099588No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAAGG773250.069.806191
GTAAGGT775750.069.8021552
GGTCAGC774550.069.733416
AAGGTCA775250.069.666614
GTCAGCT775250.069.635487
TAAGGTC776750.069.6266863
GTTTCCG398600.069.6235051
CCGTAGT401050.069.598955
GCTATCG776050.069.5974820
CTATCGG775800.069.5806621
CATGGGT642850.069.5778962
AGGTCAG776600.069.577055
CGTAGTG401450.069.56386
GGTTATC390500.069.5587717
TATCGGG776200.069.5139122
TGGGTGG642900.069.490834
AAGCTAT778750.069.4779918
GGTGGTT835100.069.462946
TCAGCTA777650.069.420578
AGCTATC778400.069.4148119
Produced by FastQC (version 0.11.5)