FastQCFastQC Report
Wed 8 May 2019
HCGVJBGXB_n01_AL234.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGVJBGXB_n01_AL234.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4914118
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA2341934.765717876534508No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAACTGTAGGC1832253.7285429450412058No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA1637413.3320526694719175No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT956731.946900745973133No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAACTGTAGG676991.3776429463028768No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA636091.294413361665308No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAACTGTAGGCA617081.2557289019107802No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC580571.1814327616878553No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGAACTG422990.8607648412187091No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA392820.7993703040911919No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAAACTGTAG351140.7145534559813175No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGA350960.7141871644107854No Hit
CAAATAAAGTATAGGCGATAGAAATTGAAACCAACTGTAGGCACCATCAA274280.5581469553641162No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTAACTGTAGGCACCATCA237260.4828129890246836No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC204250.41563918489543794No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTAAC203190.4134821345356379No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA195100.3970193633933902No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGAA146800.29873112530061346No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC135150.27502392087450894No Hit
GAGAAAGCTCACAAGAACTGCTAACTCATGCCCCCATGTCTAACAACATG132520.2696719940384012No Hit
TATAACTGAACTCCTCACACCCAATTGGACCAACTGTAGGCACCATCAAT127370.2591919852148442No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAACTGTAGGCACCATC123600.25152021176536665No Hit
CCAAATAAAGTATAGGCGATAGAAATTGAAACCAACTGTAGGCACCATCA117380.2388628030503134No Hit
GTTCTTGTAGTTGAAATACAACGATGGTTTTTCATATCATTGGTCGTGGT115510.23505744062311895No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG112440.2288101343923772No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAACTGTAGGCACCATCAAT108020.21981564138264487No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAAAACTGTA102020.2076059223649086No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA91990.18719534207359284No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT91080.1853435346892362No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTAACTGTAGGCACCATC89490.1821079591495361No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAACTGTAGGCACCATCAA79980.16275555450642415No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT77200.157098384694873No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGAACTGTAGGCACCATCA76860.1564065006172013No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC76330.15532797543730126No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC70260.14297580969769144No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG70100.1426502171905518No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG68790.13998442853834606No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTGAACTGTAGGCACCATC65600.1334929279272496No Hit
CCCAAATAAAGTATAGGCGATAGAAATTGAAACCAACTGTAGGCACCATC64470.13119343084557594No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGAACTGTAGGCACCATCAAT62580.12734736935498903No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA61410.12496647414653046No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATA57690.11739644835553399No Hit
TAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGAACTGTAGGCACC57450.11690805959482455No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCAACTGTAGGCACCATCA55760.11346898873816216No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCAAACTGTAGGCACCATCAA53720.10931768427213184No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCAACTGTAGGCACCATCAAT53360.10858510113106766No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAACTGTAGGCACC52410.10665189561992611No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAACTGTAGGCACCATCA50150.10205290145657879No Hit
TATAACTGAACTCCTCACACCCAATTGGACCAAACTGTAGGCACCATCAA50080.10191045473470518No Hit
GTTTCCGTAGTGTAGCGGTTATCACATTCGCCTAACTGTAGGCACCATCA49990.10172730894943914No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCAACTGTAGGCACCATCAA49240.10020109407222211No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGGT651750.069.84022
AGTAAGG649450.069.779731
GGTCAGC651650.069.7381446
GTCAGCT651950.069.6309057
AAGCTAT652200.069.5673718
AAGGTCA654300.069.557324
GCTATCG651200.069.5566720
AGGTCAG654400.069.52535
CTATCGG650950.069.52495621
CATGGGT428450.069.508642
TAAGGTC654800.069.50423
GGTTATC303550.069.4825817
CCGTAGT321200.069.456695
AGCTATC652250.069.4439919
TGGGTGG429700.069.4367454
TATCGGG651550.069.42940522
GGTGGTT570950.069.426656
GGCCCAT629800.069.4149727
ATGGGTG429350.069.411843
TCAGCTA653800.069.4017648
Produced by FastQC (version 0.11.5)