FastQCFastQC Report
Wed 8 May 2019
HCGVJBGXB_n01_AL233.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGVJBGXB_n01_AL233.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7204084
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA4250965.9007640666044425No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAACTGTAGGC2971454.124674281976723No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA2897054.021399528378625No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT1792192.4877416754163333No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC1117191.5507731447884283No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAACTGTAGGCA1087461.5095048863949947No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA1067761.4821592863159285No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAACTGTAGG1028641.4278567545853158No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGAACTG668310.9276821314132372No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGA652080.9051532436323619No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA634270.8804311554390537No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAAACTGTAG531590.7379008906614637No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTAACTGTAGGCACCATCA428130.5942879066929259No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC353570.49079105685053087No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTAAC347710.4826567819031538No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA332770.46191854509192287No Hit
CAAATAAAGTATAGGCGATAGAAATTGAAACCAACTGTAGGCACCATCAA276780.3841987406032467No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGAA270850.3759672985489897No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC263050.3651401066395117No Hit
GAGAAAGCTCACAAGAACTGCTAACTCATGCCCCCATGTCTAACAACATG249420.3462202828284623No Hit
GTTCTTGTAGTTGAAATACAACGATGGTTTTTCATATCATTGGTCGTGGT241540.3352820427968358No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAACTGTAGGCACCATC212310.29470783516683036No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAACTGTAGGCACCATCAAT200880.2788418347148645No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAACTGTAGGCACCATCAA195250.2710268231186644No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG160560.22287358115202432No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTAACTGTAGGCACCATC157040.21798746377749068No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAAAACTGTA156450.21716848387664553No Hit
TATAACTGAACTCCTCACACCCAATTGGACCAACTGTAGGCACCATCAAT154020.21379539716638507No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA151470.21025573827290187No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGAACTGTAGGCACCATCA150560.20899256588346277No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT145190.2015384606842452No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA141660.19663846229444298No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC131280.1822299684456761No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT130460.18109172519365405No Hit
CCAAATAAAGTATAGGCGATAGAAATTGAAACCAACTGTAGGCACCATCA125900.17476198223118997No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG121670.16889031277258845No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC118190.164059719459129No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAACTGTAGGCACCATCA118020.16382374219956347No Hit
GAGCCCAGCGCCGAATCCCCGCCCCGCGGCGGGGCGCGGGACATGTGGCG110280.1530798363816968No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTGAACTGTAGGCACCATC103910.14423762965562312No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCAACTGTAGGCACCATCA103310.14340476873950944No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG103050.14304386234252683No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATA102380.1421138343195332No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAACTGTAGGCACC101120.14036482639569445No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG100060.13889343877722693No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC98530.136769643441137No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT96960.13459032404397284No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAACTGTAGGCACCATCAAT91270.1266920263561613No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGAACTGTAGGCACCATCAAT89370.12405463345513462No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAACTGTAGGCACCAT89110.12369372705815201No Hit
GTTTCCGTAGTGTAGCGGTTATCACATTCGCCTAACTGTAGGCACCATCA84150.11680874348494548No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAACTGTAGGCACCATCAAT80200.11132574245386367No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGAACTGTAGGCACCATCA79340.11013197514076739No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATT78620.10913254204143094No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCAACTGTAGGCACCATCAA78510.10897985087347678No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCAAACTGTAGGCACCATCAA78260.10863282549176273No Hit
TAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGAACTGTAGGCACC76390.10603707563654173No Hit
CCCAAATAAAGTATAGGCGATAGAAATTGAAACCAACTGTAGGCACCATC76220.10580109837697617No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCAACTGTAGGCACCATCAAT74010.10273339400262407No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT72720.10094274303297963No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGGT1089600.069.828392
AGTAAGG1085550.069.797031
GGTCAGC1090500.069.699196
AAGGTCA1091950.069.661114
CCGTAGT540550.069.62225
GCTATCG1088400.069.6169620
CTATCGG1087950.069.6044221
GTCAGCT1091500.069.600867
AGGTCAG1093150.069.578245
TGGGTGG790750.069.5619354
TAAGGTC1093750.069.5501563
GTTTCCG537450.069.5376051
CATGGGT789800.069.53582
TATCGGG1088450.069.5306522
GGTTATC524000.069.5089117
CGTAGTG541300.069.493416
ATGGGTG790700.069.482733
AAGCTAT1092200.069.46477518
TCAGCTA1094000.069.45148
GGCCCAT1057950.069.44906627
Produced by FastQC (version 0.11.5)