FastQCFastQC Report
Wed 8 May 2019
HCGVJBGXB_n01_AL230.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCGVJBGXB_n01_AL230.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4472595
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC423610.9471235379013749No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA256960.5745210554499122No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG250950.5610836661937868No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA163750.3661185508636485No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC162260.36278715153059915No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG148310.3315972047547341No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT145460.3252250650908477No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG137840.3081879758842462No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC118090.2640301659327527No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC117240.26212970322597956No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT114800.25667425733830135No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC106630.23840745696849366No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA104360.2333321036221701No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC97700.21844141935498299No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG89380.1998392432133918No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG78600.17573690441455128No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA75790.16945419828980715No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG75540.16889523867016798No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT74620.1668382672698959No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG73750.16489308779355163No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT64430.1440550731734038No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAACTGTAGGCACCATCA62970.1407907489947111No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA61750.13806302605087203No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG61420.13732519935294835No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAACTGTAGGCACCATC57990.1296562733714991No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA55720.12458092002517554No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG52950.11838764743957367No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT49910.1115906984647615No Hit
CGTCCATGATGTTCCGCAACTACCTACATTGTTTGATCCTCATGAAAGCA46630.10425714825509574No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTA46630.10425714825509574No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACA189150.069.3973718
CAGCACG130100.069.394544
AGTAGGT223600.069.3602757
GTAGGTT223350.069.312578
TTGGCGA117300.069.3016817
TAGGTTG223300.069.296759
GCTTATC312950.069.260243
TGTATAG302600.069.2577414
AGCTTAT312900.069.23932
GCACGTA130750.069.236896
GAGGTAG482550.069.212662
GTTGTAT234200.069.2093112
TGAGGTA471150.069.208891
TAGTAGG223950.069.095626
ATTGGCG127950.069.085316
GGTAGTA437050.069.0655064
AGGTTGT267750.069.0191410
GTAGTAG434200.068.994995
TAGTTTG133800.068.966299
ACAGTGG98100.068.9161454
Produced by FastQC (version 0.11.5)